Keywords

• Bifendate Impurity B
• 128142-41-6
• Bifendate Impurity 2

Quick Details

• ProName: Bifendate Impurity B;128142-41-6
• CasNo: 128142-41-6
• Molecular Formula: C18H16O9
• Appearance: White to off white powder
• Application: experiment research
• DeliveryTime: prompt delivery
• PackAge: Food bags, aluminum foil bags
• Port: Shenzhen/hongkong
• ProductionCapacity: 5000 Gram/Year
• Purity: 99%+ HPLC
• Storage: cool and dry
• Transportation: DHL,EMS,TNT,UPS,EMS,by air,by sea;
• LimitNum: 10 Milligram
• Moisture Content: 0.1%
• Impurity: 0.1%

Superiority

• Advantages

• High-Purity Reference Standard：Confirmed by HPLC with a purity of ≥99.0%. The structure is verified through multiple methods, including NMR (1H, 13C), HRMS, and elemental analysis, providing a reliable standard for Bicyclol impurity analysis and quality control.

• Good Stability：Stable for 36 months under storage conditions of -20℃ in the dark and sealed. The degradation rate is less than 0.3% within 6 months in common solvent systems such as methanol - water mixtures, ensuring stable experimental data.

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Details

Bicyclol Impurity；128142-41-6

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• Product Information

• Product Code：B057002

• English Name：Bicyclol Impurity 2

• English Alias：5'-(hydroxymethyl)-7,7'-dimethoxy-[4,4'-bibenzo[d][1,3]dioxole]-5-carboxylic acid

• CAS No.：128142-41-6

• Molecular Formula：C??H??O?

• Molecular Weight：376.31

• Applications

• Quality Control Testing：Used for the UPLC-MS/MS detection of Impurity 2 in Bicyclol active pharmaceutical ingredients and formulations. Strictly control the impurity content to meet the ICH Q3A standards (single impurity limit ≤0.1%).

• Process Optimization Research：Monitor the formation pathway of this impurity during the synthesis of Bicyclol. By adjusting parameters such as the methoxylation reaction temperature (e.g., 50 - 60℃), reaction time, and catalyst dosage, the generation of impurities can be reduced by more than 40%.

• Method Validation：As a standard substance for the development of impurity detection methods, it can verify the resolution (≥3.0) and limit of detection (0.01 ng/mL) of UPLC, ensuring the accuracy and reliability of the detection method.

• Background Description

• Bicyclol is a drug used for the treatment of chronic hepatitis. It reduces serum alanine aminotransferase and aspartate aminotransferase levels by inhibiting liver inflammatory responses, protecting liver cell membranes, and enhancing liver detoxification functions. Impurity 2, as a process-related impurity in the synthesis of Bicyclol, may originate from side reactions during the formation of the biphenyl structure or subsequent functional group modification. Functional groups such as hydroxyl, methoxy, and carboxyl in its structure may affect drug stability, solubility, and efficacy. With the increasing requirements of regulatory agencies for the quality of drugs for liver disease treatment, the study of Impurity 2 has become an important part of ensuring the quality and safety of Bicyclol.

• Research Status

• Detection Technology：UPLC-MS/MS technology is used, combined with a C18 column (1.7μm) and gradient elution with 0.1% formic acid - acetonitrile. Impurities can be separated within 7 minutes, and the limit of detection is as low as 0.005 ng/mL, enabling precise detection of trace impurities.

• Formation Mechanism：Studies have shown that Impurity 2 may be formed by the coupling reaction of precursor compounds under alkaline conditions (such as potassium carbonate/acetone system), followed by methoxylation, hydroxymethylation, and oxidation reactions. Optimizing the polarity of the reaction solvent and the strength of the base can effectively inhibit the generation of this impurity.

• Safety Evaluation：In vitro cytotoxicity experiments show that the half-maximal inhibitory concentration (IC??) of this impurity against HepG2 cells is 198.6 μM (Bicyclol IC?? = 15.2 μM). Although the toxicity is lower than that of the main drug, its content in drugs still needs to be strictly controlled. Currently, long-term stability tests are being carried out to monitor its degradation characteristics under different humidity, light, and temperature conditions.