N-Nitrososarcosine Methyl Ester

Product Information

• Product Code：L030047

• English Name：N-Nitrososarcosine Methyl Ester

• English Alias：methyl 2-(methyl(nitroso)amino)acetate

• CAS No.：51938-19-3

• Molecular Formula：CHNO

• Molecular Weight：132.12

Applications

• Quality Control Testing：Used for UPLC-MS/MS detection of N-nitroso impurities in food, pharmaceuticals, and cosmetics, controlling content to meet international regulatory standards (e.g., ≤10 μg/kg in food).

• Environmental Monitoring：Detects N-Nitrososarcosine Methyl Ester in drinking water, soil, and industrial wastewater via solid-phase extraction coupled with GC-MS, achieving an LOD of 0.1 ng/L.

• Process Optimization Research：Monitors N-Nitrososarcosine Methyl Ester formed by nitrosation side reactions during amino acid ester synthesis, reducing generation by >70% by adjusting reaction temperature (e.g., 5-10℃) and avoiding nitrite exposure.

Background Description

N-nitroso compounds (NOCs) are known carcinogens prevalent in the environment, food processing, and chemical products. N-Nitrososarcosine Methyl Ester, as a type of NOC, may form during production of sarcosine ester-containing raw materials through reactions between amino groups and nitrites or spontaneous nitrosation under acidic or high-temperature storage conditions. Its nitrosamide group is genotoxic. With stricter regulations on NOCs by WHO, FDA, studying such impurities is crucial for ensuring food and environmental safety.

Research Status

• Detection Technology：GC-MS/MS with a DB-5MS column (30m×0.25mm×0.25μm) and selected ion monitoring (SIM) mode achieves separation within 6 minutes, with an LOD of 0.05 ng/L for trace analysis.

• Formation Mechanism：Formed by reaction of sarcosine methyl ester with sodium nitrite under acidic conditions (pH<4); optimizing reaction pH (neutral) and adding antioxidants (e.g., vitamin C) inhibits its formation.

• Safety Evaluation：Animal studies show carcinogenicity, with a permitted daily intake (TTC value) determined as 1.5 μg/day. Accelerated stability testing is ongoing to monitor nitrosation rates under different humidity and light conditions.